RNA

Part:BBa_K3201002:Design

Designed by: Anastasios Galanis, Nikolaos Karvelas   Group: iGEM19_Athens   (2019-10-15)


S.mansoni Type 1 Hammerhead Ribozyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part had to be validated in vitro, so that the cleavage activity was assessed. Therefore that was possible through the addition of a T7 promoter and T7 terminator, and the use of an in vitro transcription kit. Afterwards the hammerhead was activated and the cleavage was confirmed using PAGE electrophoresis in TBE buffer.


Source

S.mansoni genome

References

1.Klauser, B., Atanasov, J., Siewert, L. K. & Hartig, J. S. Ribozyme-Based Aminoglycoside Switches of Gene Expression Engineered by Genetic Selection in S. cerevisiae. ACS Synth. Biol. 4, 516–525 (2015).