RNA
Part:BBa_K3201002:Design
Designed by: Anastasios Galanis, Nikolaos Karvelas Group: iGEM19_Athens (2019-10-15)
S.mansoni Type 1 Hammerhead Ribozyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part had to be validated in vitro, so that the cleavage activity was assessed. Therefore that was possible through the addition of a T7 promoter and T7 terminator, and the use of an in vitro transcription kit. Afterwards the hammerhead was activated and the cleavage was confirmed using PAGE electrophoresis in TBE buffer.
Source
S.mansoni genome
References
1.Klauser, B., Atanasov, J., Siewert, L. K. & Hartig, J. S. Ribozyme-Based Aminoglycoside Switches of Gene Expression Engineered by Genetic Selection in S. cerevisiae. ACS Synth. Biol. 4, 516–525 (2015).